DNA fingerprinting Analysis of Mycobacterium tuberculosis isolates

in Ho Chi Minh city, 1999 ? 2000.

Hoang Thi Kim Lien¹, Nguyen Thi Ngoc Lan², Pham Hung Van³, Hoang Thi Thuy², Pham Thi Hoang Anh², Mimount Enaimi⁴, Anne Marie van den Brandt⁴, Kristin Kremmer⁴, Martien Borgdorff⁵, Dick Van Soolingen⁴.

In order to study DNA fingerprinting of Mycobacterium tuberculosis isolates in Ho Chi Minh city, a cross ? sectional study was performed with 95 patients randomly collected. All of isolates had been confirmed by culture and subjected to susceptibility test. Subsequently, restriction fragment length polymorphism (RFLP) of IS6110 and spoligotyping from all of them were analysed. The patients? treatment histories were used to classify their cases according to WHO and national tuberculosis program criteria.

Analysis of population structure of Mycobacterium tuberculosis strains showed that 42 isolates (44%) were found in the family Beijing genotype; 13 isolates (14%) belonged to Viet Nam genotype; two smaller clusters consisted of 4 and 5 strains with identical RFLP patterns for each; and 31 strains (33%) with unique RFLP patterns. The association between the Beijing genotype and chronic cases as well as treatment failure are sixfold higher than the other genotypes (p < 0,05). The multidrug ? resistant cases correlate with male sex and the Beijing genotype, odds ratio 3,02 and 2,6 respectively (p < 0,05). The other genotypes which are different from Beijing and Viet Nam is at risk of multidrug ? resistant tuberculosis less than three times (p < 0,05).

1: University Training Center for Health care Professionals, HCMC

2: Pham Ngoc Thach Center for tuberculosis and lung disease

3: University of Medicine and Pharmacy

4: The national Institute of public health and environment, The Netherlands

5: Royal Tuberculosis Association, the Hague, The Netherlands

Correspondent: Hoang Thi Kim Lien, kimlien901@yahoo.com